Research Journal of Engineering and Technology

D. Suresh ¹, P. Sridhar ¹, D. Joyce Hellen Sathya ², M. Anil Kumar ², M. Seenuvasan ², R. Dhandapani ³

Affiliations
1 Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode, IN
2 Department of Biotechnology, Madha Engineering College, Kunrathur, Chennai, IN
3 Department of Microbiology, Periyar University, Salem, IN

Abstract

A bacterium designated as Acinetobacter sp., isolated from domestic sewage soil identified by taxonomic studies by 16S rDNA sequential analysis was found to decolorize azo dye with the decolorization efficiency varying from 80-96%. This strain decolorized 96.2% a deep red sulphonated azo dye Reactive RedC2G29 (200 mg/L) with 1.76 g/L dry cells of specific decolorization rate in static condition at optimum pH 5.0 and temperature 30°C. The degradation efficiency of this strain was within 48 h. Phytotoxicity studies revealed less toxic nature of decolorized products as compared to original dye. HPLC, GC-MS and LC-MS analysis revealed the production of metabolites as final product by degradation of the dye.

Keywords

Reactive Red C2G29, Degradation, Acinetobacter sp.

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M. Anil Kumar ¹, S. Keerthana ¹, Adyasa Pani ¹, D. Suresh ¹, M. Seenuvasan ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai, IN

Abstract

The combined effects of initial sucrose and initial Reactive Red C2G 29 dye concentrations on the specific growth rate and dye bioaccumulation efficiency of Penicillium chrysogenum MTCC 6477 was determined at pH 5.0. The highest growth rate of 0.115 h^-1 was obtained at an initial sucrose concentration of 15 g/L in absence of dye. For each constant sucrose concentration chosen between 5 and 15 g/L with an increase in initial dye concentration up to 500 mg/L under pH 5.0 resulted in a decrease in percentage uptake of dye and more over all dye concentration tested inhibited the growth of Penicillium chrysogenum. On the other hand at each constant dye concentration studied, both the specific growth rate and uptake yield enhanced with raising sucrose concentration up to15 g/L with pH 5.0, the maximum uptake yield of 96.67 % was observed in 15g/L sucrose and 50 mg/L dye containing growth medium at pH 5.0. To check the stability of this bioaccumulation process a continuous bioaccumulation system was developed and it shows monotonic damping oscillation where the dye uptake concentration approaches equilibrium.

Keywords

Penicillium chrysogenum, Mycelium, Bioaccumulation, Stability, Reactive Red.

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M. B. Nishita ¹, Remya Rachel Chacko ¹, M. Anil Kumar ¹, D. Suresh ¹, S. Selvanaveen ¹, M. Seenuvasan ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai 600 069, IN

Abstract

A novel fibrinolytic enzyme from two different blood fibrin Bos taurus and Ovis aries as an added ingredient by Rhizomucor sp. which was purified by ammonium sulphate precipitation, dialysis and ion exchange chromatography. The purification protocol resulted in 1.373 and 1.737 -fold purification of the enzyme. The apparent molecular weight of the enzyme was found to be 30.0kDa, determined by sodium dodecylsulphate poly acrylamide gel electrophoresis. Optimal temperature and pH of the nattokinase were to be at 37°C and 8.0 respectively. Upon the addition of zinc chloride the enzyme activity was increased while EDTA and PMSF inhibited the activity of the enzyme, indicating the presence of metalloproteases. The produced enzyme showcases the blood clot-busting activity.

Keywords

Nattokinase, Bos taurus, Ovis aries, Rhizomucor sp.

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